human thyroid cancer tissue array  (Novus Biologicals)

Journal: Oncogene

Article Title: Peroxisome proliferator-activated receptor gamma is frequently downregulated in a diversity of sporadic nonmedullary thyroid carcinomas.

doi: 10.1038/sj.onc.1206400

Figure Lengend Snippet: Figure 2 Immunohistochemistry with anti-PPARg antibody. Except for four frozen FTC tumor sections, all tissues were paraffin embedded and most were analysed as components of tissue microarrays. Normal thyroid sections were included in each experiment as positive controls. Following dewaxing with xylene and antigen retrieval in citric acid buffer for 30 min at 941C, the PPARg (E8) mouse monoclonal antibody (sc-7273, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) was incubated at a dilution of one in 12 for 1 h at 371C. Detection was by the LSAB2 streptavidin-horseradish peroxidase system (DAKO Corp., Car- pinteria, CA, USA) and counterstaining with hematoxylin l. Slides were scored for the presence or absence of nuclear staining and the intensity of staining was compared to the normal control from the same experiment. (a) normal thyroid shows moderate staining in the nucleus; (b) FTC with complete absence of staining; and (c) translocation-positive FTC shows very strong nuclear staining

Article Snippet: A total of 142 sections were analysed, as detailed in Table 1b, including 17 of the 19 FTCs used for RT–PCR analysis, additional independent cases accrued through the Department of Pathology, The Ohio State University and a commercial human thyroid cancer tissue array (Imgenex IMH-319).

Techniques: Immunohistochemistry, Incubation, Staining, Control, Translocation Assay